UFM1 Protein (AA 1-83) (His tag)

Catalog No. ABIN667370

This Recombinant UFM1 protein is produced in Escherichia coli (E. coli).

Quick Overview for UFM1 Protein (AA 1-83) (His tag) (ABIN667370)

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-83

Purification tag / Conjugate: This UFM1 protein is labelled with His tag.

Sequence: MSKVSFKITL TSDPRLPYKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGIGINPA QTAGNVFLKH GSELRIIPRD RVG

Characteristics: UFM1, 1-83aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Alternative Name: UFM1

Background: UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation. It localizes primarily to the nucleus, but is also present in diffuse amounts in the cytoplasm. This protein is expressed in a variety of tissues, including kidney, brain, heart, liver and lung. Recombinant human UFM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 11.1 kDa (103aa) confirmed by MALDI-TOF

NCBI Accession: NP_057701