HchA (AA 1-283) protein (His tag)

Catalog No. ABIN667426

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for HchA (AA 1-283) protein (His tag) (ABIN667426)

Target: HchA

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-283

Purification tag / Conjugate: His tag

Characteristics: hchA, 1-283aa, E.coli, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Storage: 4 °C

Target Details

Target: HchA

Background: HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Hsp31 molecular chaperone, ECK1963, JW1950, yedU, yzzC. NCBI no.: NP_416476

Molecular Weight: 33.3 kDa(303aa) confirmed by MALDI-TOF