HchA (AA 1-283) protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
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- Target
- HchA
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-283
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Characteristics
- hchA, 1-283aa, E.coli, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
- Storage
- 4 °C
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- Target
- HchA
- Background
- HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Hsp31 molecular chaperone, ECK1963, JW1950, yedU, yzzC. NCBI no.: NP_416476
- Molecular Weight
- 33.3 kDa(303aa) confirmed by MALDI-TOF
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