MsrB (AA 1-137) protein (His tag)

Catalog No. ABIN667429

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for MsrB (AA 1-137) protein (His tag) (ABIN667429)

Target: MsrB

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-137

Purification tag / Conjugate: His tag

Characteristics: msrB, 1-137aa, E.coli, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Storage: 4 °C

Target Details

Target: MsrB

Background: MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E.coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Methionine sulfoxide reductase B, ECK1776, JW1767, yeaA. NCBI no.: NP_416292

Molecular Weight: 17.6 kDa (157aa) confirmed by MALDI-TOF