MsrB (AA 1-137) protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
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- Target
- MsrB
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-137
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Characteristics
- msrB, 1-137aa, E.coli, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
- Storage
- 4 °C
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- Target
- MsrB
- Background
- MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E.coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Methionine sulfoxide reductase B, ECK1776, JW1767, yeaA. NCBI no.: NP_416292
- Molecular Weight
- 17.6 kDa (157aa) confirmed by MALDI-TOF
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