GrxB (AA 1-215) protein (His tag)

Catalog No. ABIN667442

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for GrxB (AA 1-215) protein (His tag) (ABIN667442)

Target: GrxB

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-215

Purification tag / Conjugate: His tag

Characteristics: grxB, 1-215aa, E.coli, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Storage: 4 °C

Target Details

Target: GrxB

Background: GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E.coli grxB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2. NCBI no.: NP_415582

Molecular Weight: 26.5kDa (235aa) confirmed by MALDI-TOF