CysH (AA 1-244) protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 90 % by SDS - PAGE
SDS
1 image
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- Target
- CysH
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-244
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Characteristics
- cysH, 1-244aa, E.coli, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
- Storage
- 4 °C
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- Target
- CysH
- Background
- CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3',5'-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3'-phosphoadenylyl sulfate and thioredoxin. Recombinant E.coli cysH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Phosphoadenosine phosphosulfate reductase. NCBI no.: NP_417242
- Molecular Weight
- 30.1kDa (264aa) confirmed by MALDI-TOF
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