CysH (AA 1-244) protein (His tag)

Catalog No. ABIN667443

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for CysH (AA 1-244) protein (His tag) (ABIN667443)

Target: CysH

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-244

Purification tag / Conjugate: His tag

Characteristics: cysH, 1-244aa, E.coli, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Storage: 4 °C

Target Details

Target: CysH

Background: CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3',5'-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3'-phosphoadenylyl sulfate and thioredoxin. Recombinant E.coli cysH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Phosphoadenosine phosphosulfate reductase. NCBI no.: NP_417242

Molecular Weight: 30.1kDa (264aa) confirmed by MALDI-TOF