UBE2D3 Protein (AA 1-149) (His tag)
Quick Overview for UBE2D3 Protein (AA 1-149) (His tag) (ABIN667596)
Target
See all UBE2D3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-149
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Purification tag / Conjugate
- This UBE2D3 protein is labelled with His tag.
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Sequence
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MLSNRKCLSK ELSDLARDPP AQCSAGPVGD DMFHWQATIM GPNDSPYQGG VFFLTIHFPT DYPFKPPKVA FTTRIYHPNI NSNGSICLDI LRSQWSPALT ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDRDKYNRIS REWTQKYAM -
Characteristics
- UBE2D3, 1-149aa, Human, His tag, E.coli
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Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (His tag)
custom-made UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (GST tag)UBE2D3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147), (full length) protein (His-SUMO Tag)UBE2D3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 9) protein (Myc-DYKDDDDK Tag)UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))
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Alternative Name
- UbcH5c/UBE2D3
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Background
- UBE2D3, also known as ubiquitin-conjugating enzyme E2 D3. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Recombinant human UBE2D3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 19 kDa (169aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_871622
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Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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