UBE2D3 Protein (AA 1-147, full length) (His-SUMO Tag)

Catalog No. ABIN5710584

Product Details

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Protein Type: Recombinant

Protein Characteristics: full length, AA 1-147

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2D3 protein is labelled with His-SUMO Tag.

Application: SDS-PAGE (SDS)

Sequence: MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD RDKYNRISRE WTQKYAM

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Alternative Name: UB2D3 (UBE2D3 Products)

Synonyms: E2(17)KB3 Protein, UBC4/5 Protein, UBCH5C Protein, eff Protein, fb36f02 Protein, wu:fb36f02 Protein, zgc:56340 Protein, 1100001F19Rik Protein, 9430029A22Rik Protein, AA414951 Protein, UBE2D3P Protein, Xubc4 Protein, e2(17)kb3 Protein, ubc4/5 Protein, ubch5c Protein, ube2d3.1 Protein, ube2d2 Protein, xubc4 Protein, wu:fj13d01 Protein, zgc:73200 Protein, ubiquitin conjugating enzyme E2 D3 Protein, ubiquitin-conjugating enzyme E2D 3 Protein, ubiquitin conjugating enzyme E2 D3 L homeolog Protein, ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast), like Protein, UBE2D3 Protein, ube2d3 Protein, Ube2d3 Protein, LOC100223566 Protein, ube2d3.L Protein, ube2d2l Protein

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma mbrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction .

Molecular Weight: 32.7 kDa

UniProt: P61077

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades