GCSH Protein (AA 49-173) (His tag)

Catalog No. ABIN667601

This Recombinant GCSH protein is produced in Escherichia coli (E. coli).

Quick Overview for GCSH Protein (AA 49-173) (His tag) (ABIN667601)

Target: GCSH (Glycine Cleavage System H Protein (GCSH))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 49-173

Purification tag / Conjugate: This GCSH protein is labelled with His tag.

Sequence: SVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE

Characteristics: GCSH, 49-173 aa , Human,His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT, 0.15M NaCl.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GCSH (Glycine Cleavage System H Protein (GCSH))

Alternative Name: GCSH

Background: The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 16.4 kDa (149aa) confirmed by MALDI-TOF

NCBI Accession: NP_004474