R-Ras Protein (AA 1-215) (His tag)

Catalog No. ABIN667781

This Recombinant R-Ras protein is produced in Escherichia coli (E. coli).

Quick Overview for R-Ras Protein (AA 1-215) (His tag) (ABIN667781)

Target: R-Ras

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-215

Purification tag / Conjugate: This R-Ras protein is labelled with His tag.

Sequence: MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPC

Characteristics: RRAS, 1-215 aa, Human, His-tagged, Recombinant, E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: R-Ras

Alternative Name: RRAS

Background: RRAS, also known as ras-related protein R-Ras, belongs to the Ras family which functions in signal transduction pathways. This protein has been shown to interact with RASSF5, NCK1, Bcl-2, ARAF and RALGDS. RRAS can bind GTP and GDP, and it has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant RRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 25.3 kDa (235aa) confirmed by MALDI-TOF

NCBI Accession: NP_006261