ETHE1 Protein (AA 13-254) (His tag)
Quick Overview for ETHE1 Protein (AA 13-254) (His tag) (ABIN668013)
Target
See all ETHE1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 13-254
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Purification tag / Conjugate
- This ETHE1 protein is labelled with His tag.
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Sequence
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LSQRG GSGAPILLRQ MFEPVSCTFT YLLGDRESRE AVLIDPVLET APRDAQLIKE LGLRLLYAVN THCHADHITG SGLLRSLLPG CQSVISRLSG AQADLHIEDG DSIRFGRFAL ETRASPGHTP GCVTFVLNDH SMAFTGDALL IRGCGRTDFQ QGCAKTLYHS VHEKIFTLPG DCLIYPAHDY HGFTVSTVEE ERTLNPRLTL SCEEFVKIMG NLNLPKPQQI DFAVPANMRC GVQTPTA -
Characteristics
- ETHE1, 13-254aa, Human, His tag, E.coli
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Purification
- > 90% by SDS-PAGE
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Ethylmalonic Encephalopathy 1 (ETHE1) (AA 155-254) protein (GST tag)
ETHE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ethylmalonic Encephalopathy 1 (ETHE1) (AA 1-254) protein (GST tag)ETHE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ethylmalonic Encephalopathy 1 (ETHE1) (AA 13-254) protein (His-IF2DI Tag)ETHE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ethylmalonic Encephalopathy 1 (ETHE1) (AA 8-254) protein (His-SUMO Tag)ETHE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Ethylmalonic Encephalopathy 1 (ETHE1) protein (Myc-DYKDDDDK Tag)ETHE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.1M NaCl
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ETHE1 (Ethylmalonic Encephalopathy 1 (ETHE1))
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Alternative Name
- ETHE1
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Background
- ETHE1 is a mitochondrial sulfur dioxygenase involved in catabolism of sulfide that accumulates to toxic levels in ethylmalonic encephalopathy. Mutations of ETHE1 were detected in all the typical ethylmalonic encephalopathy patients analysed, but no ETHE1 mutations were identified in patients presenting with early onset progressive encephalopathy with ethylmalonic aciduria. Recombinant human ETHE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 29.1 kDa (267aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_055112
Target
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