BAX Protein (GST tag)

Catalog No. ABIN6699545

Supplier Product No.: 009-001-r47s

Product Details

Target: BAX (BCL2-Associated X Protein (BAX))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BAX protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r47s

Supplier: Rockland

Purpose: BAX recombinant protein-GST fusion protein

Purification: Recombinant full-length human BAX was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Purity: >75%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: BAX Protein is suitable for use in Western Blot. Expect a band approximately ~ 49 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: BAX Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX (BAX Products)

Background:

Synonyms: BCL2-associated X protein, BCL2L4, Apoptosis regulator BAX

Background: BAX is a proapoptotic protein of the BCL2 protein family. BAX forms a heterodimer with BCL2 and functions as an apoptotic activator. BAX interacts with and increases the opening of the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in the mitochondrial membrane potential and the release of cytochrome c (1). The expression of BAX gene is regulated by the tumor suppressor p53 and BAX has been shown to be involved in p53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene (2). BAX Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and Neurobiology research.

NCBI Accession: NM_004324

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response