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PPP3CA Protein (GST tag)

PPP3CA Origin: Human Host: Insect cells (Sf9) Recombinant >85% WB
Rockland
Catalog No. ABIN6699565
Supplier Product No.: 009-001-t02s
  • Target See all PPP3CA Proteins
    PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))
    Protein Type
    Recombinant
    Origin
    • 4
    • 1
    Human
    Source
    • 3
    • 1
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This PPP3CA protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-t02s
    Supplier
    Rockland
    Purpose
    PP2B recombinant protein-GST fusion protein
    Purification
    Recombinant full-length human PP2B was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
    Purity
    >85%
    Top Product
    Discover our top product PPP3CA Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: PP2AB Protein is suitable for use in Western Blot. Expect a band approximately ~83 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    PP2B Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))
    Alternative Name
    PPP3CA (PPP3CA Products)
    Background

    Synonyms: PPP2B, PPP3CA, CALN, CCN1, CNA1, CALNA, CALNA1, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform

    Background: PP2B is a key regulator of the anaphase-promoting factor which is an excellent substrate for protein phosphatase. PP2B is involved in bovine ocular tissues and is postulated to be involved in the immunologic privilege of the cornea, in retinal signal transduction, and in the toxic effects of immunosuppressants on the eye. PP2B acts as an inhibitory constraint on I-LTP that is relieved by PKA, and that this inhibitory constraint acts as a gate to regulate the synaptic induction of L-LTP (1). PP2B plays a role in the transition from short- to long-term memory and also regulates bone formation through an effect on osteoblast differentiation (2). PP2B Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Metabolic Disorder, Phosphatases, and Ser/Thr Kinases research.

    NCBI Accession
    NM_001130691
    Pathways
    RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling
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