CDC7 Protein (GST tag)

Catalog No. ABIN6699595

Supplier Product No.: 009-001-r61s

This Recombinant CDC7 protein is produced in Insect cells (Sf9).

Quick Overview for CDC7 Protein (GST tag) (ABIN6699595)

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >70%

Product Details

Purification tag / Conjugate: This CDC7 protein is labelled with GST tag.

Supplier Product No.: 009-001-r61s

Supplier: Rockland

Purpose: CDC7 recombinant protein-GST fusion protein

Purification: Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

Application Notes:

Application Note: CDC7 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CDC7 Protein is suitable for use in Western Blot. Expect a band approximately ~ 94 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: CDC7 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Alternative Name: CDC7

Background:

Synonyms: CDC7L1, HsCDC7, Hsk1, huCDC7, MGC117361, MGC126237, MGC126238, Cell division cycle 7-related protein kinase, CDC7-related kinase,

Background: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the cell division cycle. Overexpression of CDC7 gene product may be associated with neoplastic transformation for some tumors. Inhibition of CDC7 in cancer cells impairs progression through S phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability (1). Inhibition of CDC7 kinase activity in cancer cells restricts DNA replication and induces apoptosis. CDC7 phosphorylates the minichromosome maintenance protein 2 (Mcm2), a component of the DNA replicative helicase needed for genome duplication (2). CDC7 Protein is ideal for investigators involved in Signaling Proteins, Cell-Cycle Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

NCBI Accession: NM_003503

Pathways: Mitotic G1-G1/S Phases, DNA Replication