CCL2 Protein

Catalog No. ABIN6699608

Supplier Product No.: 009-001-b60

This Recombinant CCL2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL2 Protein (ABIN6699608)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-b60

Supplier: Rockland

Purpose: MCP-1 Human Recombinant Protein

Purification: Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non- reducing SDS-PAGE, against known standard.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: MCP-1 activity is determined by the ability to chemoattract human monocytes or THP-1 cells at concentrations of 4 - 15 ng/mL.

Application Details

Application Notes:

Application Note: MCP-1 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MCP-1 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: CCL2

Background:

Synonyms: CCL2, C-C motif chemokine 2, Monocyte Chemotactic and Activating Factor, MCAF, Small-inducible cytokine A2, SCYA2, Monocyte chemotactic protein 1, Monocyte secretory protein JE, HC11

Background: MCP-1, or Monocyte chemoattractant protein 1, of the intercrine beta (chemokine CC) family, is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. It augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. MCP-1 exists as a monomer or homodimer, in equilibrium. It binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Recombinant Human MCP-1 produced in E. coli is a non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8607 Daltons.

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection