CCL19 Protein

Catalog No. ABIN6699625

Supplier Product No.: 010-001-c21

This Recombinant CCL19 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL19 Protein (ABIN6699625)

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 010-001-c21

Supplier: Rockland

Purpose: MIP-3ß Mouse Recombinant Protein

Purification: Purity was determined to be greater than 98% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Recombinant mouse MIP-3-beta induces chemotaxis of human T- cells in a concentration range of 8 - 80 ng/ml.

Application Details

Application Notes:

Application Note: MIP-3ß protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-3ß in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 μg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Alternative Name: Ccl19

Background:

Synonyms: C-C motif chemokine 19, Small-inducible cytokine A19, Epstein-Barr virus-induced molecule 1 ligand chemokine, EBI1 ligand chemokine, ELC

Background: Macrophage Inflammatory Protein-3-beta (CCL 19, of the intercrine beta chemokine CC family) is an important secreted chemoattractant for dendritic cells, antigen-engaged B-cells, and effector-memory T-cells, signaling through the CCR7 receptor. It may play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. MIP-3ß specifically binds to chemokine receptor CCR7. MIP-3ß is strongly chemotactic for naive CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory CD4 T-cells. There is significant expression in dendritic cells in mesenteric and peripheral lymph nodes, and in spleen (T-cell zone or periarteriolar lymphatic sheath) and Peyer patches, but low expression in thymus. Recombinant mouse MIP-3-beta is a polypeptide chain consisting of 83 amino acids with a molecular weight of 9,200 Daltons.

UniProt: O70460

Pathways: Positive Regulation of Immune Effector Process