CCL5 Protein

Catalog No. ABIN6699635

Supplier Product No.: 009-001-c20-0020

This Recombinant CCL5 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL5 Protein (ABIN6699635)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-c20-0020

Supplier: Rockland

Purpose: Human RANTES (CCL5) Recombinant Protein

Purification: RANTES (CCL5) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human monocytes, neutrophils, THP-1 or primary T cells at 1-8 ng/mL.

Application Details

Application Notes:

Application Note: RANTES Recombinant Protein has been tested by biologic activity and is suitable as a control for polyclonal or monoclonal anti-RANTES in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: CCL5

Background:

Synonyms: EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228 (TCP228), T-cell-specific protein RANTES

Background: Regulated Upon Activation Normal T cell Express Sequence (RANTES), also called CCL5, is a chemokine produced by T cells three to five days after activation. RANTES is a promiscuous chemokine that signals through several G protein-coupled receptors, CCR5, CCR3, CCR1 and US28 (a viral receptor encoded by human CMV). The main function of RANTES is to recruit immune cells to the site of inflammation. Recombinant human RANTES is a non-glycosylated protein, containing 68 amino acids, with a molecular weight of 7.8 kDa.

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration