Cofilin Protein (His tag)

Catalog No. ABIN6699707

Supplier Product No.: 009-001-r72s

This Recombinant Cofilin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Cofilin Protein (His tag) (ABIN6699707)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >95%

Product Details

Purification tag / Conjugate: This Cofilin protein is labelled with His tag.

Supplier Product No.: 009-001-r72s

Supplier: Rockland

Purpose: Cofilin 1 recombinant protein-HIS Epitope

Purification: Recombinant full-length human Cofilin 1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: Cofilin 1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol. Cofilin 1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 23 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Cofilin 1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1

Background:

Synonyms: CFL1, CFL, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform

Background: Cofilin 1 (non-muscle), also known as CFL1, is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner (1). It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus (2). CFL1 processed proteins are most probably produced by HIV-1 protease digestion. Cofilin 1 Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cancer, Cardiovascular Disease, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_005507

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events