DNMT1 Protein (GST tag)

Catalog No. ABIN6699781

Supplier Product No.: 010-001-r85s

This Recombinant DNMT1 protein is produced in Insect cells (Sf9).

Quick Overview for DNMT1 Protein (GST tag) (ABIN6699781)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Protein Type: Recombinant

Origin: Mouse

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >70%

Product Details

Purification tag / Conjugate: This DNMT1 protein is labelled with GST tag.

Supplier Product No.: 010-001-r85s

Supplier: Rockland

Purpose: DNMT1 recombinant protein-GST fusion protein

Purification: Recombinant mouse DNMT1 (766-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

Application Notes:

Application Note: DNMT1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. DNMT1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 94 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.05 μg/μL

Buffer: DNMT1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background:

Synonyms: Dnmt, MCMT, Cxxc9, MTase, Met-1, Dnmt1o, m.MmuI, Met1, MommeD2, Cytosine-specific methyltransferase, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Background: DNMT1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1). DNMT1 enzyme is crucial for stable expression and function in vivo (2). DNMT1 is essential for maintenance of DNA methylation and the interaction of DNMT1 with the replication machinery is not strictly necessary for maintenance of DNA methylation, but improves its efficiency. DNMT1 is necessary and sufficient to maintain global methylation and aberrant CpG island methylation in human cancer cells. DNMT1 Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection