HSPA1AB protein (GST tag)

Catalog No. ABIN6699935

Supplier Product No.: 009-001-s14s

This Recombinant HSPA1AB protein is expressed in Insect cells (Sf9).

Quick Overview for HSPA1AB protein (GST tag) (ABIN6699935)

Target: HSPA1AB

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: GST tag

Supplier Product No.: 009-001-s14s

Supplier: Rockland

Purpose: HSP70 recombinant protein-GST fusion protein

Purification: Recombinant full-length human HSP70 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: HSP70 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. HSP70 Protein is suitable for use in Western Blot and ATPase Assay. Expect a band approximately ~70 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: ATPase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: HSP70 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: HSPA1AB

Background:

Synonyms: HSPA1A, HSP72, HSPA1, HSPA1B, HSP70-1, Heat shock 70 kDa protein 1A/1B , Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2

Background: HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli. HSP70 Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Apoptosis/Autophagy, Cancer, and Cellular Stress research.

NCBI Accession: NM_005345