HSPA90AA1 protein (His tag)

Catalog No. ABIN6699937

Supplier Product No.: 009-001-f69s

This Recombinant HSPA90AA1 protein is produced in Insect cells (Sf9).

Quick Overview for HSPA90AA1 protein (His tag) (ABIN6699937)

Target: HSPA90AA1

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: His tag

Supplier Product No.: 009-001-f69s

Supplier: Rockland

Purpose: HSP90 alpha recombinant protein-HIS Epitope

Purification: Recombinant full length human HSP90α was expressed by baculovirus in Sf9 insect cells using a C-Terminal his epitope. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: HSP90α Protein is stored in 50 mM MOPS, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. HSP90α Protein is suitable for use in Western Blot. Expect a band approximately ~94 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: HSP90α Protein is stored in 50 mM MOPS, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: HSPA90AA1

Background:

Synonyms: HSP90AA1, HSPN, LAP2, HSP86, HSPC1, HSPCA, Hsp89, Hsp90, HSP90N, HSPCAL1, HSPCAL4, FLJ31884, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38

Background: HSP90α is a member of the HSP90 family of proteins which are important molecular chaperones involved in signal transduction, cell cycle control, stress management, folding, degradation, and transport of proteins (1). HSP90 is a molecular chaperone that plays a key role in the conformational maturation of oncogenic signaling proteins, including HER2/ERBB2, AKT, RAF1, BCR-ABL and mutated p53. HSP90 inhibitors bind to HSP90, and induce the proteasomal degradation of HSP90 client proteins. HSP90α is an important mediator of cancer cell invasion and is expressed extracellularly on fibrosarcoma and breast cancer cells where it interacts with MMP2 (2). HSP90α Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, and Cellular Stress research.

Gene ID: 3320

NCBI Accession: NM_005348

UniProt: P07900