IGF2 Protein

Catalog No. ABIN6699973

Supplier Product No.: 009-001-v23-0050

This Recombinant IGF2 protein is produced in Escherichia coli (E. coli).

Quick Overview for IGF2 Protein (ABIN6699973)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-v23-0050

Supplier: Rockland

Purpose: Human Insulin-like Growth Factor II Recombinant Protein

Purification: Insulin-like Growth Factor II purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose dependent proliferation of FDC-P1 cells and is typically 1.5-6 ng/mL.

Application Details

Application Notes:

Application Note: Insulin-like Growth Factor II Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Insulin-like Growth Factor II in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: IGF2

Background:

Synonyms: Somatamedin A

Background: Insulin-like Growth Factor II, IGF-II, is a major growth hormone made by Theca cells during gestation. While IGF-II is known to engage the IGF-I receptor (IGF1R) to mediate embryonic growth, IGF-II is also known to bind the IGF-II receptor (IGF2R). IGF2R is thought to be signaling dead receptor that acts as a sink by binding up free IGF-II. Recombinant human IGF-II is non-glycosylated protein, containing 67 amino acids, with a molecular weight of 7.5 kDa.

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation