Interferon gamma Protein (IFNG)

Catalog No. ABIN6699975

Supplier Product No.: 009-001-v20-0020

Recombinant Interferon gamma protein expressed in Escherichia coli (E. coli).

Quick Overview for Interferon gamma Protein (IFNG) (ABIN6699975)

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-v20-0020

Supplier: Rockland

Purpose: Human Interferon-gamma Recombinant Protein

Purification: Interferon-gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured in antiviral assays using virally infected L929 cells and is typically less than 0.1 ng/mL.

Application Details

Application Notes:

Application Note: Lyophilized from an aqueous solution containing 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5. Interferon-gamma Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer: Lyophilized from an aqueous solution containing 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Background:

Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFN-γ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Human IFNγ does not show cross-reactivity with mouse. Recombinant human IFNγ is a non-glycosylated protein, containing 144 amino acids, with a molecular weight of 16.88 kDa.

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy