IFNG1-2 Protein

Catalog No. ABIN6699978

Supplier Product No.: 010-001-v20-0020

Recombinant IFNG1-2 protein expressed in Escherichia coli (E. coli).

Quick Overview for IFNG1-2 Protein (ABIN6699978)

Target: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-v20-0020

Supplier: Rockland

Purpose: Mouse Interferon gamma Recombinant Protein

Purification: Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by Viral CPE assay using EMC virus on L929 cells and is typically greater than 1.0 x 10^7units/mg.

Application Details

Application Notes:

Application Note: Interferon-gamma Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays. Product is provided as a dried powder, lyophilized from 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer: Product is provided as a dried powder, lyophilized from 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Alternative Name: INF gamma

Background:

Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Human IFNγ does not show cross-reactivity with mouse. Recombinant mouse IFNγ is a non-glycosylated protein, containing 134 amino acids, with a molecular weight of 15.6 kDa.

UniProt: P01580