IFNG1-2 Protein

Catalog No. ABIN6699982

Supplier Product No.: 012-001-v20-0100

This Recombinant IFNG1-2 protein is produced in Escherichia coli (E. coli).

Quick Overview for IFNG1-2 Protein (ABIN6699982)

Target: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 012-001-v20-0100

Supplier: Rockland

Purpose: Rat Interferon gamma Recombinant Protein

Purification: Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined in a viral challenge assay using EMC virus on L929 and is typically less than 0.1 ng/mL.

Application Details

Application Notes:

Application Note: Interferon-gamma Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays. Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer: Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Alternative Name: INF gamma

Background:

Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Recombinant rat IFNγ is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.6 kDa.

UniProt: P01581