IL1A Protein

Catalog No. ABIN6699986

Supplier Product No.: 009-001-v24-0100

This Recombinant IL1A protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (ABIN6699986)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-v24-0100

Supplier: Rockland

Purpose: Human Interleukin-1-alpha Recombinant Protein

Purification: Interleukin-1-alpha purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the stimulation of D10S.G4.1 cells and is typically 1-6 pg/mL.

Application Details

Application Notes:

Application Note: Interleukin-1-alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-alpha in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A

Background:

Synonyms: IL-1F1, FAF, BAF, LEM, LAF, Hematopoietin-1

Background: Interleukin-1 alpha (IL-1α) is constitutively expressed by epithelial cells, but can also be produced by most other cells upon stimulation. IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1α is a non-glycosylated single chain protein, containing 159 amino acids, with a molecular weight of 18 kDa.

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints