IL16 Protein

Catalog No. ABIN6700048

Supplier Product No.: 010-001-v55-0010

This Recombinant IL16 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL16 Protein (ABIN6700048)

Target: IL16 (Interleukin 16 (IL16))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-v55-0010

Supplier: Rockland

Purpose: Mouse Interleukin-16 Recombinant Protein

Purification: Interleukin-16 purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract primary human T cells and is typically less than 200 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-16 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-16 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: Il16

Background:

Synonyms: Lymphocyte Chemoattractant Factor (LCF)

Background: Interleukin 16 (IL-16) is produced primarily by CD4+ and CD8+ T cells and acts as a chemo-attractant for lymphocytes, monocytes, eosinophils, dendritic cells and Langerhans cells. Additionally, IL-16 has been reported to upregulate IL-2 receptor (CD25), induce progression of cells to the G1 phase and suppress HIV & SIV replication. Recombinant mouse IL-16 is a non-glycosylated protein, containing 127 amino acids, with a molecular weight of 13.2 kDa.

UniProt: O54824