IL-25 Protein

Catalog No. ABIN6700092

Supplier Product No.: 009-001-v57-0025

Recombinant IL-25 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-25 Protein (ABIN6700092)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-v57-0025

Supplier: Rockland

Purpose: Human Interleukin-17E (IL-25) Recombinant Protein

Purification: Interleukin-17E (IL-25) purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependant production of IL-8 from cultured human PBMCs and is typically 10-100 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-17E Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-17E in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: 0.01M HCl

Reconstitution_Volume: 25 μL (25-250 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL25

Background:

Synonyms: IL17E

Background: Interleukin-17E (IL-17E), also commonly called IL-25, is a pro-inflammatory cytokine member of a six-species family of proteins (IL-17A-17F). IL-17F stimulates secretion of IL-8 and induces activation of NF-kB by binding to the receptor IL-17RB. Recombinant human IL-17E is a non-glycosylated, disulfide-linked, homodimer, containing two 145 amino acid chains, with a total molecular weight of 35.5 kDa.

UniProt: Q9H293