IL-27 Protein

Catalog No. ABIN6700106

Supplier Product No.: 010-f01-b54-0100

This Recombinant IL-27 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-27 Protein (ABIN6700106)

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 010-f01-b54-0100

Supplier: Rockland

Purpose: Mouse Interleukin-27 (p28) Recombinant Protein (Animal Free)

Purification: The p28 subunit of IL-27 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein (with a 50% confidence range).

Biological Activity Comment: Mouse p28 biological activity was measured via dose-dependent inhibition of TGFß and IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of Mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. no acceptance criteria limits established.

Application Details

Application Notes:

Application Note: Interleukin-27 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-27 in immunological assays. Buffer Formulation: 10 mM sodium bicarbonate, pH 8.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer: Buffer Formulation: 10 mM sodium bicarbonate, pH 8.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Alternative Name: Il27

Background:

Synonyms: IL-30, P28, IL-27 p28 subunit

Background: The p28 subunit of IL-27 (IL-27/p28), also known as Interleukin-30 (IL-30), is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), p28 forms a heterodimer known as IL-27. p28 is thought to be a pro-inflammatory cytokine inducing immuno-modulatory effects, but the details of these effects are currently unclear and research is focused on delineating its specific biological functions. Recombinant mouse p28 is a non-glycosylated protein, containing 207 amino acids, with a molecular weight of 23.7 kDa.

UniProt: Q8K3I6