IL32 Protein

Catalog No. ABIN6700125

Supplier Product No.: 009-001-b49

Recombinant IL32 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL32 Protein (ABIN6700125)

Target: IL32 (Interleukin 32 (IL32))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-b49

Supplier: Rockland

Purpose: IL-32A Human Recombinant Protein

Purification: Purity was determined to be greater than 97% as determined by reducing and non-reducing SDS- PAGE.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 for this assay is in the range of 0.125- 1.0ug/ml.

Application Details

Application Notes:

Application Note: IL-32A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-32A in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.05 M Potassium Phosphate, pH 7.8

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL32 (Interleukin 32 (IL32))

Alternative Name: IL32

Background:

Synonyms: Interleukin-32 alpha, IL-32a cytokine, Natural killer cells protein 4 alpha, Tumor necrosis factor alpha-inducing factor

Background: Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-a, and MIP-2 production from human & mouse macrophage cell lines. It is upregulated in activated T- & NK-cells, and IFN-g-treated epithelial cells. Human recombinant IL-32 alpha produced in E. coli is a 131 amino acid non -glycosylated polypeptide with a molecular mass of 14.9 kDa.

UniProt: P24001-4