Quick Overview for IL-33 Protein (ABIN6700126)
Target
See all IL-33 (IL33) ProteinsProtein Type
Origin
Source
Application
Purity
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Supplier Product No.
- 009-001-b50
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Supplier
- Rockland
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Purpose
- IL-33 Human Recombinant Protein
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Purification
- Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
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Endotoxin Level
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
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Biological Activity Comment
- Assay data for Human recombinant IL-33 is based upon qualitative binding to anti-IL-33 antibody.
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Interleukin 33 (IL33) (AA 112-270) protein
IL33 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 33 (IL33) (AA 1-266) protein (His tag)custom-made IL33 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin 33 (IL33) (AA 1-270) protein (His tag)custom-made IL33 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin 33 (IL33) (AA 109-270) (Active) protein (His tag)IL33 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 33 (IL33) (AA 109-264) protein (His tag)IL33 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 33 (IL33) (AA 109-270) protein (His tag)IL33 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 33 (IL33) protein (His tag)IL33 Origin: Rhesus Monkey Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 33 (IL33) (AA 171-269) protein (GST tag)IL33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
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Application Note: IL-33 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-33 in immunological assays.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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Concentration
- 0.1 mg/mL
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Buffer
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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Preservative
- Without preservative
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Storage
- -20 °C
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Storage Comment
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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Expiry Date
- 6 months
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- IL-33 (IL33) (Interleukin 33 (IL33))
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Alternative Name
- IL33
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Background
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Synonyms: Interleukin-33, IL-33 cytokine, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV
Background: Recombinant Human IL-33 produced in E. coli is a non-disulfide-linked homodimeric, non-glycosylated polypeptide chain consisting of 160 amino acids and having a total molecular mass of 18,125 Daltons.
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UniProt
- O95760
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Pathways
- Production of Molecular Mediator of Immune Response
Target
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