IL-4 Protein

Catalog No. ABIN6700135

Supplier Product No.: 009-001-b93

This Recombinant IL-4 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-4 Protein (ABIN6700135)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-b93

Supplier: Rockland

Purpose: IL-4 Human Recombinant Protein

Purification: Purity was determined to be greater than 98% as determined by analysis by RP-HPLC, and by reducing and non-reducing SDS-PAGE, against known standard.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Recombinant Human Interleukin-4 is fully biologically active when compared to standard. The ED50 as measured by CD23 receptor binding on human proliferal macrophages at a concentration of 1.8 ng/ml.

Application Details

Application Notes:

Application Note: IL-4 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-4 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Background:

Synonyms: B Cell Growth/Stimulatory Factor 1, BSF1, B cell IgG differentiation factor, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra, IL4e12 cytokine, HCGF, IA Inducing Factor, MFF, MCGF2, MGC79402, T cell growth factor 2, IGG1 induction factor

Background: Interleukin (IL)-4, secreted primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes. Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses. It participates in several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Recombinant Human IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15,000 Daltons.

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation