NCK1 Protein (His tag)

Catalog No. ABIN6700285

Supplier Product No.: 009-001-s55s

This Recombinant NCK1 protein is produced in Insect cells (Sf9).

Quick Overview for NCK1 Protein (His tag) (ABIN6700285)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >75%

Product Details

Purification tag / Conjugate: This NCK1 protein is labelled with His tag.

Supplier Product No.: 009-001-s55s

Supplier: Rockland

Purpose: NCK recombinant protein-HIS Epitope

Purification: Recombinant full-length human NCK was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >75% by densitometry.

Application Details

Application Notes:

Application Note: NCK Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. NCK Protein is suitable for use in Western Blot. Expect a band approximately ~48 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: NCK Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background:

Synonyms: NCK1, MGC12668, NCKalpha, Cytoplasmic protein NCK1 NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha

Background: NCK (or non-catalytic region of tyrosine kinase adaptor protein 1) is an adaptor protein that is located in the cytoplasm and involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS (1). NCK is a signaling and transforming protein that contains Src homology 2 and 3 (SH2 and SH3) domains. NCK functions by coupling tyrosine phosphorylation via SH2 domains to downstream effectors through SH3 domains. Furthermore, NCK couples tyrosine phosphorylation guidance signals to cytoskeletal events required for the ipsilateral projections of spinal cord neurons and for normal limb movement (2). NCK Protein is ideal for investigators involved in Signaling Proteins, Adaptor Proteins, Angiogenesis, Cancer, Inflammation, and Invasion/Metastasis research.

NCBI Accession: NM_006153

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals