NCK1 Protein (His tag)
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- Target See all NCK1 Proteins
- NCK1 (NCK Adaptor Protein 1 (NCK1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NCK1 protein is labelled with His tag.
- Purpose
- Recombinant Human NCK1 Protein (His Tag)
- Sequence
- Met 1-Ser377
- Characteristics
- Recombinant Human Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus.
- Purity
- > 80 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product NCK1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 5 % Mannitol, pH 8.0.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- NCK1 (NCK Adaptor Protein 1 (NCK1))
- Alternative Name
- NCK1 (NCK1 Products)
- Synonyms
- NCK Protein, NCKalpha Protein, nck-1 Protein, NCK1 Protein, 6330586M15Rik Protein, D230010O13Rik Protein, Nck Protein, Nck-1 Protein, Nck1 Protein, nck Protein, nck1-a Protein, nck1-b Protein, nckalpha Protein, NCK adaptor protein 1 Protein, non-catalytic region of tyrosine kinase adaptor protein 1 Protein, NCK adaptor protein 1b Protein, NCK adaptor protein 1 L homeolog Protein, NCK1 Protein, Nck1 Protein, nck1b Protein, nck1.L Protein
- Background
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Background: Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
Synonym: Cytoplasmic Protein NCK1, NCK adaptor Protein 1, Nck-1, SH2/SH3 Adaptor Protein NCK-Alpha, NCK1, NCK
- Molecular Weight
- 45.0 kDa
- UniProt
- P16333
- Pathways
- TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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