NAMPT Protein
Quick Overview for NAMPT Protein (ABIN6700315)
Target
See all NAMPT ProteinsProtein Type
Origin
Source
Application
Purity
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Supplier Product No.
- 009-001-w40-0100
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Supplier
- Rockland
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Purpose
- Human Visfatin Recombinant Protein
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Purification
- Visfatin purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.
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Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100 ng/mL.
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Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) protein (His tag)
custom-made NAMPT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) protein (His tag)custom-made NAMPT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) (Active) protein (His tag)NAMPT Origin: Human Host: Escherichia coli (E. coli) Recombinant >90 % (SDS-PAGE) SDS Active
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-368) protein (GST tag)NAMPT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 27-491) protein (His tag)NAMPT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nicotinamide phosphoribosyltransferase (NAMPT) proteinNAMPT Origin: Human Host: Escherichia coli (E. coli) Recombinant 90,00% SDS
Nicotinamide phosphoribosyltransferase (NAMPT) proteinNAMPT Origin: Human Host: Escherichia coli (E. coli) Recombinant 90,00% SDS
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Application Notes
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Application Note: Visfatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Visfatin in immunological assays.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: 0.02M HCl
Reconstitution_Volume: 100 μL
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Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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Preservative
- Without preservative
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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Expiry Date
- 6 months
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- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
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Alternative Name
- NAMPT
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Background
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Synonyms: Pre-B-cell colony-enhancing factor 1, PBEF
Background: Visfatin is an adipokine produced by visceral adipose tissue. Other adipokines include leptin, adiponectin, resistin, chemerin, omentin and vaspin. Visfatin has been shown to act as a pro-inflammatory factor for PBMCs and may mimic the effects of insulin on adipocytes, monocytes and hepatocytes. Recombinant human Visfatin is a non-glycosylated protein, containing 466 amino acids, with a molecular weight of 52.6 kDa.
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UniProt
- P43490
Target
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