p73 alpha Protein (GST tag)

Catalog No. ABIN6700342

Supplier Product No.: 009-001-s70s

This Recombinant p73 alpha protein is produced in Insect cells (Sf9).

Quick Overview for p73 alpha Protein (GST tag) (ABIN6700342)

Target: p73 alpha (TP73 alpha) (Tumor Protein P73 alpha (TP73 alpha))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This p73 alpha protein is labelled with GST tag.

Supplier Product No.: 009-001-s70s

Supplier: Rockland

Purpose: p73 alpha recombinant protein-GST fusion protein

Purification: Full-length recombinant human p73α was expressed by baculovirus in Sf9 cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: p73α Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. p73α Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: p73α Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: p73 alpha (TP73 alpha) (Tumor Protein P73 alpha (TP73 alpha))

Alternative Name: p73 alpha

Background:

Synonyms: TP73, TAp73alpha, Tumor protein p73, p53-like transcription factor, p53-related protein

Background: p73α is a member of the p53 family of transcription factors that are involved in cellular responses to stress and development. P73 has high sequence similarity to p53 and p63, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. P73 is a stress-response gene and a downstream effector in the p53 pathway (1). The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. p73 is the components of a mismatch repair-dependent apoptosis pathway which contributes to cisplatin-induced cytotoxicity (2). p73α Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, and p38 Pathway research.

NCBI Accession: NM_005427