Prolactin Protein (PRL)
Quick Overview for Prolactin Protein (PRL) (ABIN6700415)
Target
See all Prolactin (PRL) ProteinsProtein Type
Origin
Source
Application
Purity
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Supplier Product No.
- 009-001-w15-0010
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Supplier
- Rockland
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Purpose
- Human Prolactin Recombinant Protein
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Purification
- prolactin purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the ability to stimulate proliferation of NB2-11 cells and is typically less than 1 ng/mL.
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Prolactin (PRL) (Active) protein
PRL Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Prolactin (PRL) protein (His tag)PRL Origin: Sheep Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Prolactin (PRL) (AA 29-227) protein (Fc Tag)PRL Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Prolactin (PRL) protein (His tag)PRL Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Prolactin (PRL) (AA 29-227) protein (GST tag)PRL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prolactin (PRL) (AA 29-227) protein (Fc Tag,AVI tag,Biotin)PRL Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Application Notes
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Application Note: Prolactin Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Prolactin in immunological assays.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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Buffer
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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Preservative
- Without preservative
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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Expiry Date
- 6 months
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- Prolactin (PRL)
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Alternative Name
- PRL
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Background
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Synonyms: LTH, Mammotropin, Luterotropic hormone, Lutetropin
Background: Prolactin (PRL) is a hormone that is made and secreted by the pituitary gland and acts in an endocrine, paracrine and autocrine manner. The prolactin receptor is expressed on many cell types and is linked to JAK kinase signaling pathways. PRL's primary function is to regulate lactation, but it is known to have many other regulation functions. These functions include roles in the immune system, cell growth, apoptosis, and differentiation. Recombinant human Prolactin is a non-glycosylated protein, containing 200 amino acids, with a molecular weight of 23 kDa.
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UniProt
- P01236
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Pathways
- JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
Target
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