SCYL3 Protein (GST tag)

Catalog No. ABIN6700491

Supplier Product No.: 009-001-t27s

Recombinant SCYL3 protein expressed in Insect cells (Sf9).

Quick Overview for SCYL3 Protein (GST tag) (ABIN6700491)

Target: SCYL3 (SCY1-Like 3 (SCYL3))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: This SCYL3 protein is labelled with GST tag.

Supplier Product No.: 009-001-t27s

Supplier: Rockland

Purpose: SCYL3 recombinant protein-GST fusion protein

Purification: Recombinant human SCYL3 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: SCYL3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SCYL3 Protein is suitable for use in Western Blot. Expect a band approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: SCYL3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SCYL3 (SCY1-Like 3 (SCYL3))

Alternative Name: SCYL3

Background:

Synonyms: PACE-1, PACE1, RP1-97P20.2, Protein-associating with the carboxyl-terminal domain of ezrin, Ezrin-binding protein PACE-1, SCY1-like protein 3, SCYL3

Background: SCYL3 encodes a protein with a kinase domain and four HEAT repeats which interact with the C-terminal domain of ezrin, an ERM protein which play a role in cell adhesion and migration (1). C-terminal domain of SCYL3 interacts with the C-terminal alpha-helical domain of ezrin. Biochemical assays indicate that SCYL3 had associated rather than intrinsic protein kinase activity. SCYL3 is associated with the cytoplasmic face of the Golgi apparatus. This distribution is dependent upon the presence of the SCYL3 N-terminal myristoylation consensus sequence but is not dependent on an association with ezrin. SCYL3 is highly expressed in breast carcinoma cells and adult colon. SCYL3 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Inflammation, Invasion/Metastasis, and Neurobiology research.