SUMO2 Protein (His tag)

Catalog No. ABIN6700552

Supplier Product No.: 009-001-t67

This Recombinant SUMO2 protein is produced in Escherichia coli (E. coli).

Quick Overview for SUMO2 Protein (His tag) (ABIN6700552)

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >95%

Product Details

Purification tag / Conjugate: This SUMO2 protein is labelled with His tag.

Supplier Product No.: 009-001-t67

Supplier: Rockland

Purpose: SUMO2 recombinant protein-HIS Epitope

Purification: Recombinant human SUMO2 (1-93) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: SUMO2 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. SUMO2 Protein is suitable for use in Western Blot. Expect a band approximately ~17.5 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: SUMO2 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Alternative Name: SUMO2

Background:

Synonyms: HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A, Small ubiquitin-related modifier 2

Background: SUMO2 is a member of the SUMO (small ubiquitin-like modifier) protein family which functions in a manner similar to ubiquitin. SUMO2 is bound to target proteins as part of a post-translational modification system and modifies numerous cellular proteins and affect their metabolism and function. SUMO2 is also involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (1). SUMO2 is predominantly detected on nuclear bodies (2). SUMO2 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Apoptosis/Autophagy, Cell Cycle, and NfkB Pathway research.

Pathways: Methionine Biosynthetic Process