TGFB1 Protein

Catalog No. ABIN6700722

Supplier Product No.: 009-001-w26-0005

Product Details

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Supplier Product No.: 009-001-w26-0005

Supplier: Rockland

Purpose: Human Transforming Growth Factor beta 1 Recombinant Protein

Purification: Transforming Growth Factor beta 1 purity was determined to be greater than 98% as determined by reducing and non-reducing SDS-pAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured by the dose-dependent inhibition of IL-4-induced proliferation from mouse HT-2 cells and is typically 0.04-0.2 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Transforming Growth Factor beta 1 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Transforming Growth Factor beta 1 in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative, it does contain Trehalose in a ratio of 20:1 (Trehalose:Protein) DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1 (TGFB1 Products)

Background:

Synonyms: Differentiation inhibiting factor, cartilage-inducing factor

Background: Transforming Growth Factor-beta 1 (TGF-β1) is a member of the TGF-beta superfamily. Members of this family are known to exhibit regulatory activity in immunity and proliferation pathways. TGF-β1 signals through SMAD proteins via the TGF-β1 RI and RII receptors. Mature human TGF-β1 is a glycosylated homodimer, containing two 112 amino acids chains, with a total molecular weight of 25 kDa.

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints