TNF alpha Protein

Catalog No. ABIN6700748

Supplier Product No.: 010-001-321

Product Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 010-001-321

Supplier: Rockland

Purpose: TNF-α Mouse Recombinant Protein

Purification: Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is 0.04 EU/μg protein (with 50% confidence range).

Biological Activity Comment: Recombinant Mouse TNFα is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is 13-20 pg/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: TNFα protein has been tested by SDDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

References

Sun, Yau, Wong, Li, Huang, Yao: "Role of TRPM2 in H(2)O(2)-induced cell apoptosis in endothelial cells." in: PloS one, Vol. 7, Issue 8, pp. e43186, (2013) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tnf (TNF alpha Products)

Background:

Synonyms: Tumor necrosis factor, TNF-alpha, TNF-a, TNFα, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin

Background: Tumor Necrosis Factor-alpha (TNF-a) is pleiotrophic cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following stimulation by bacterial LPS . TNF-a activity is mediated via interactions with TNFR1 &TNFR2 receptors. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 & GM-CSF. Recombinant mouse TNF-a produced in E.Coli is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 17,300 daltons.

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome