Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin) Protein

TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Pubmed (2 references)
Catalog No. ABIN2181833
$1,661.00
Plus shipping costs $45.00
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target See all TNF alpha Proteins
    TNF alpha
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 77-233
    Origin
    • 37
    • 22
    • 11
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 59
    • 26
    • 11
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This TNF alpha protein is labelled with Biotin.
    Brand
    MABSol®,UltraLys
    Sequence
    AA 77-233
    Specificity
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
    Characteristics
    The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.
    Purity
    >95 % as determined by reduced SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
  • Comment

    A chemically labeled biotinylated protein with ultra sensitivity.
    The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
    Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • Gao, Lu, Guo, Xin: "Dual-optofluidic waveguide in-line fiber biosensor for real-time label-free detection of interferon-gamma with temperature compensation." in: Optics express, Vol. 28, Issue 7, pp. 10491-10504, (2021) (PubMed).

    El Amrani, van den Broek, Göbel, van Maarseveen et al.: "Quantification of active infliximab in human serum with liquid chromatography-tandem mass spectrometry using a tumor necrosis factor alpha -based pre-analytical sample purification and a stable ..." in: Journal of chromatography. A, Vol. 1454, pp. 42-8, (2016) (PubMed).

  • Target
    TNF alpha
    Alternative Name
    TNF-alpha (TNF alpha Products)
    Synonyms
    DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa
    Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
    Molecular Weight
    17.4 kDa
    NCBI Accession
    NP_000585
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
You are here:
help Support