RANKL Protein

Catalog No. ABIN6700759

Supplier Product No.: 009-001-c22

This Recombinant RANKL protein is produced in Escherichia coli (E. coli).

Quick Overview for RANKL Protein (ABIN6700759)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-c22

Supplier: Rockland

Purpose: RANKL Human Recombinant Protein

Purification: Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Application Details

Application Notes:

Application Note: RANKL Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-RANKL in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: TNFSF11

Background:

Synonyms: Rank Ligand, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TRANCE, Osteoprotegerin ligand, OPGL, Osteoclast differentiation factor, ODF, CD254

Background: The secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE, ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. It may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form. Defects in TNFSF11 cause osteopetrosis. Recombinant human soluble RANKL produced in E.coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20,006 Daltons.

UniProt: O14788

Pathways: NF-kappaB Signaling