BAFF Protein

Catalog No. ABIN6700762

Supplier Product No.: 009-001-u65-0020

This Recombinant BAFF protein is produced in Escherichia coli (E. coli).

Quick Overview for BAFF Protein (ABIN6700762)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 90,00%

Product Details

Supplier Product No.: 009-001-u65-0020

Supplier: Rockland

Purpose: Human BAFF

Purification: BAFF purity was determined to be greater than 90% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a RPMI-8226 cell survival assay in the presence of 0.2 μM DEX and is typically less than 30 ng/mL.

Application Details

Application Notes:

Application Note: Human BAFF Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-BAFF in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: TNFSF13B

Background:

Synonyms: THANK, B lymphocyte stimulator (BLyS), B-cell activating factor, TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1), CD_antigen=CD257

Background: Human B cell Activating Factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. BAFF initiates signaling through three receptors with human BAFF having activity on mouse splenocytes. Recombinant human BAFF is a non-glycosylated protein containing a N terminal His tag, a thrombin cut site and 153 amino acids of the TNF-like domain. This protein has a total molecular weight of 18.5 kDa.

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response