YWHAB Protein

Catalog No. ABIN6700772

Supplier Product No.: 009-001-r24s

Product Details

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r24s

Supplier: Rockland

Purpose: 14-3-3 alpha/beta recombinant protein

Characteristics: 14-3-3 alpha/beta, YWHAB, HS1, GW128, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1

Purification: Recombinant full-length human 14-3-3α/β was expressed in E.coli cells. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: 14-3-3 alpha/beta Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3 alpha/beta Protein is suitable for use in Western Blot. Expect a band approximately ~29 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Alternative Name: 14-3-3 alpha/beta (YWHAB Products)

Background:

Synonyms: 14-3-3 alpha/beta, YWHAB, HS1, GW128, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1

Background: 14-3-3α/β is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3α/β protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-β interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-β to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth. 14-3-3 alpha/beta Protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.

NCBI Accession: NM_003404

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location