YWHAE Protein

Catalog No. ABIN6700776

Supplier Product No.: 009-001-r26s

Recombinant YWHAE protein expressed in Escherichia coli (E. coli).

Quick Overview for YWHAE Protein (ABIN6700776)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Supplier Product No.: 009-001-r26s

Supplier: Rockland

Purpose: 14-3-3 epsilon recombinant protein

Purification: Recombinant full-length human tag-free 14-3-3ε was expressed in E. coli cells. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3ε Protein is suitable for use in Western Blot. Expect a band approximately ~30 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE

Background:

Synonyms: 14-3-3 epsilon, YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465

Background: 14-3-3ε also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3ε has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3ε interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3ε, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2). 14-3-3ε protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.

NCBI Accession: NM_006761

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase