14-3-3 theta Protein (YWHAQ)

Catalog No. ABIN6700780

Supplier Product No.: 009-001-r30s

This Recombinant 14-3-3 theta protein is produced in Escherichia coli (E. coli).

Quick Overview for 14-3-3 theta Protein (YWHAQ) (ABIN6700780)

Target: 14-3-3 theta (YWHAQ)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Supplier Product No.: 009-001-r30s

Supplier: Rockland

Purpose: 14-3-3 theta recombinant protein

Purification: Recombinant full-length human 14-3-3θ was expressed in E. coli cells. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: 14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3θ Protein is suitable for use in Western Blot. Expect a band approximately ~ 29 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: 14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: 14-3-3 theta (YWHAQ)

Alternative Name: YWHAQ

Background:

Synonyms: 14-3-3 theta, YWHAQ, 1C5, HS1, 14-3-3, 14-3-3 protein tau

Background: 14-3-3θ (also known as tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3θ plays an important role in controlling apoptosis (1). Induced expression of 14-3-3θ protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3θ has been observed to mediate nucleocytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2). 14-3-3θ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.

NCBI Accession: NM_006142

Pathways: Apoptosis, Myometrial Relaxation and Contraction