14-3-3 zeta Protein (YWHAZ) (GST tag)

Catalog No. ABIN6700786

Supplier Product No.: 009-001-r27s

This Recombinant 14-3-3 zeta protein is produced in Escherichia coli (E. coli).

Quick Overview for 14-3-3 zeta Protein (YWHAZ) (GST tag) (ABIN6700786)

Target: 14-3-3 zeta (YWHAZ)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This 14-3-3 zeta protein is labelled with GST tag.

Supplier Product No.: 009-001-r27s

Supplier: Rockland

Purpose: 14-3-3 zeta recombinant protein-GST fusion protein

Purification: Recombinant full-length human tag-free 14-3-3ζ was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein . The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: 14-3-3ζ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3ζ Protein is suitable for use in Western Blot. Expect a band approximately ~ 55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: 14-3-3ζ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ

Background:

Synonyms: 14-3-3 zeta, YWHAZ, KCIP-1, MGC111427, MGC126532, MGC138156, 14-3-3 protein zeta, 14-3-3-like protein, Protein Leonardo, 14-3-3, 14-3-3EZ, leo, THAP

Background: 14-3-3ζ (also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3ζ protein plays a key role in cancer biology by being an important regulator of major cellular processes such as proliferation, differentiation, senescence and apoptosis (1). 14-3-3ζ protein has been shown to interact with the IRS1 protein, suggesting a role for this protein in regulating insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (2). 14-3-3ζ Protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, WNT Signaling research.

NCBI Accession: NM_003406

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location