UBE2J1 Protein (GST tag)

Catalog No. ABIN6700800

Supplier Product No.: 009-001-u38s

Recombinant UBE2J1 protein expressed in Insect cells (Sf9).

Quick Overview for UBE2J1 Protein (GST tag) (ABIN6700800)

Target: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >70%

Product Details

Purification tag / Conjugate: This UBE2J1 protein is labelled with GST tag.

Supplier Product No.: 009-001-u38s

Supplier: Rockland

Purpose: UBE2J1 recombinant protein-GST fusion protein

Purification: Recombinant full-length human UBE2J1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: UBE2J1 Protein is suitable for use in Western Blot. Expect a band approximately ~70 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: UBE2J1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Alternative Name: UBE2J1

Background:

Synonyms: CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, Ubc6p, Ubiquitin-conjugating enzyme E2 J1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E

Background: UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family which is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. UBE2J1 play an important role in the homeostatic regulation of MHC class I assembly and expression (1). HRD1 and UBE2J1 associate in a complex together with non-β2m bound MHC class I heavy chains, Derlin 1, and p97 and discriminate misfolded MHC class I from conformational MHC I-β2m-peptide heterotrimers. UBE2J1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.