UBE2W Protein (GST tag)
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- Target See all UBE2W Proteins
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBE2W protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-u46s
- Supplier
- Rockland
- Purpose
- UBE2W recombinant protein-GST fusion protein
- Purification
- Recombinant full length human UBE2W was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
- Purity
- >75%
- Top Product
- Discover our top product UBE2W Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: UBE2W Protein is suitable for use in Western Blot. Expect a band approximately ~45 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1 μg/μL
- Buffer
- UBE2W Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- Alternative Name
- UBE2W (UBE2W Products)
- Background
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Synonyms: hUBC-16, UBC-16, UBC16, FLJ11011, Homo sapiens ubiquitin-conjugating enzyme E2W
Background: UBE2W or ubiquitin-conjugating enzyme E2W (putative) encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) (1). Ubiquitin-conjugating enzyme (E2) along with ubiquitin-activating (E1) and ligating (E3) enzymes coordinates the addition of an ubiquitin moiety to existing proteins. UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2 and may be important in the repair of DNA damage (2). UBE2W is highly expressed in heart, brain, liver, and pancreas. UBE2W Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.
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