UBE2W Protein (AA 1-151, full length) (His-SUMO Tag)
-
- Target See all UBE2W Proteins
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- Protein Type
- Recombinant
- Protein Characteristics
- full length, AA 1-151
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBE2W protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product UBE2W Protein
-
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-162) protein (His tag)
UBE2W Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (His tag)UBE2W Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-159) protein (GST tag)UBE2W Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 17-112) protein (GST tag)UBE2W Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (Strep Tag)Crystallography grade UBE2W Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2W Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2W Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
- Target
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- Alternative Name
- UBE2W (UBE2W Products)
- Synonyms
- UBE2W Protein, im:6905459 Protein, si:ch211-193n21.4 Protein, ube2wa Protein, UBC-16 Protein, UBC16 Protein, 6130401J04Rik Protein, ubiquitin conjugating enzyme E2 W Protein, ubiquitin-conjugating enzyme E2W (putative) Protein, ubiquitin conjugating enzyme E2 W (putative) Protein, ubiquitin conjugating enzyme E2W (putative) Protein, ubiquitin-conjugating enzyme E2W Protein, UBE2W Protein, ube2w Protein, Ube2w Protein
- Background
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi ania complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.
- Molecular Weight
- 33.3 kDa
- UniProt
- Q96B02
-
